ybaE [2020-04-30 12:54:15]

ATP-binding A2 component of [SW|ECF transporter]s

locus: BSU_01460

pI: 5.82

mw: 30.43 kDa

protein length: 276 aa

gene length: 831 bp

function: uptake of micronutrients

product: [SW|ECF transporter] (ATP-binding protein)

essential: no

ec: 3.6.3.-

synonyms: ecfA2

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter] → [category|SW 1.2.3.1|The general components of the ECF transporters]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 151,303 152,133

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|D8637043C7E2291589C966E95F94DC61B1A8AB75|YbxA]

[SW|Domains]

• [SW|ABC transporter domain] (aa 1-233) (according to UniProt)

Structure

• [PDB|4HUQ] (EcfA1-EcfA2-EcfT-FolT complex of ''Lactobacillus brevis''), [Pubmed|23584589]

[SW|Localization]

• membrane associated (via [protein|7DC7BD54ED425859FFD45B8B6F3522750A938835|YbaF]) [Pubmed|10092453]

Biological materials

Mutant

• BKE01460 ([gene|5ABF4F7A3D00B523F67B281241A6A8101EC60057|ybaE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTCTTCATCTGATAGCGAT, downstream forward: _UP4_CTTTTTCAGGGAGAGAAGAC
• BKK01460 ([gene|5ABF4F7A3D00B523F67B281241A6A8101EC60057|ybaE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTCTTCATCTGATAGCGAT, downstream forward: _UP4_CTTTTTCAGGGAGAGAAGAC
• BP1149 ([gene|search|ybxA-ybaE-ybaf]::cat trp⁺) available at [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs

References

• 10092453,20497229,22574898,24362466